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Antibodies: A Laboratory Manual
Ed Harlow, David Lane

Cold Spring Harbor Laboratory Press, 1988 - 726 pages

average customer review:based on 3 reviews
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An excellent summary of immunological methods.

This book is indespensible for those wishing to set up any type of antibody related methodology. Gives excellent overviews of immunodiagnostic methods (ELISA,RIA,etc.), antibody production (polyclonal and monoclonal), and includes basic summaries on the nature of the immune sytem in vivo. Especially useful to researchers developing immuno-techniques as it gives detailed methodologies.


Good manual, though needs revision.

This laboratory manual is very concise and easy to read. The topics range from antibody production to protein/enzyme conjugation, with step by step instructions and formulations. Previous to most topics is given a fairly thorough background. One drawback is the fact that this book is nearly 20 years old (pub. 1988) and lacks a significant amount of progress made in the areas of homo and heterobifunctional linkers, PEGylation, and antibody engineering. Given a good price however, this would be a good reference manual.


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An old classic, but still useful.

This classic is getting old...
But you cand find some important informations inside, classic protocols and keys-data to the antibodies-world.
A must-have reference for any today-biologist working with antibodies (immunostaining, immunoprecipitation, Western-blot, antibodies production, etc...).



Introduction to immunochemistry for molecular biologists and other nonspecialists. Spiral.



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